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Collaborative Studies on Lung Stem Cell Biology and Cell Based Therapy
 
 


Barry R. Stripp, Ph.D.
Professor of Medicine and Cell Biology
Division of Pulmonary, Allergy, and Critial Care
Duke University Medical Center

Dr. Barry Stripp is a Professor of Medicine and Cell Biology in the Division of Pulmonary, Allergy, and Critical Care at Duke University Medical Center.

He received his Ph.D. at East Carolina School of Medicine in Greenville, NC.

Along with his work at the Duke University Medical Center, he is also:

  • Member, Duke University School of Medicine Comprehensive Cancer Center

Visit the Stripp Lab Website
Email Dr. Stripp

Consortium Research Title: Stem Cells to Enhance Bronchiolar Reparative Capacity.

Consortium Research Description:

Goals of this proposal are to extend fundamental principles in lung stem cell biology for the development of effective strategies to amplify and purify lung stem cells, deliver them to repair deficient airways, and enhance epithelial reparative capacity. The underlying premise upon which this proposal is based is that defects in the reparative capacity of epithelial cells represent a common factor contributing to the progression of chronic lung disease, and that strategies aimed at enhancing epithelial reparative capacity will be essential components of treatments to effect lung regeneration. To achieve the goals of this proposal we have organized a research team drawing from basic and clinical scientists with expertise in stem cell biology, cell-based therapy, lung injury and repair, and the clinical management of chronic lung disease in humans. The scientific foundation for studies proposed in this application is based upon our previous demonstration that endogenous tissue stem cells are required for maintenance of epithelial reparative capacity, and that potentiation of b-catenin signaling in airways of mice leads to expansion of endogenous stem cells that harbor intrinsic reparative capacity. Accordingly, we hypothesize that pharmacologic interventions capable of transiently activating b-catenin signaling will lead to expansion of endogenous stem cells and enhanced epithelial reparative capacity, and that enriched populations of reparative cells obtained through potentiation of b-catenin signaling can be used for restoration of epithelial reparative capacity in repair-deficient recipient airways. Three aims are proposed that will build a platform upon which cell-based therapies can be further developed. Aim 1 will use transient activation of b-catenin signaling for the amplification of mouse and human airway stem cells. In Aim 2, we will define the cell surface phenotype of bronchiolar stem cells for the prospective purification and enrichment of airway stem cells. Finally, in Aim 3, we will test the feasibility of using amplified bronchiolar stem cells for the restoration of reparative capacity in repair-deficient airways. Accomplishing these aims will provide a rational foundation upon which to further develop strategies for the delivery of reparative cells to lung tissue for correction of epithelial repair defects.

 

Stripp Lab Information: 
106 Research Dr.
DUMC Box 10300
Durham, NC 27710
Phone: 919-668-7762
Fax: 919-684-5266

Assistant Name: Amy Loeblein
Email Amy
Phone: 919-668-7762

We are interested in understanding basic mechanisms regulating epithelial maintenance and renewal in the pulmonary system. Our goal is to understand fundamental principles of tissue maintenance and repair that will be informative for development of new treatments for patients with lung disease. Ongoing research builds upon our initial discovery of resident lung stem cells and extends these observations to understand cellular and molecular mechanisms regulating their behavior in the intact lung and in culture. We use genetically modified animal models to manipulate and tag airway epithelial cell types, coupled with analysis of cell phenotype at the molecular level, to understand cellular behavior in normal and repairing states.

Recent Publications

  • Giangreco A, Arwert EN, Rosewell IR, Snyder J, Watt FM, Stripp BR. Stem cells are dispensable for lung homeostasis but restore airways after injury. Proc. Natl. Acad. Sci. USA. (Epub 2009)
  • Snyder JC, Reynolds SD, Hollingsworth JW, Li Z, Kaminski N, Stripp BR. Clara cells attenuate the inflammatory response through regulation of macrophage behaviour. Am. J. Respir. Cell Mol. Biol. (Epub 2009)
  • Zemke, A.C., R. M. Teisanu, A. Giangreco, J. A. Drake, B. L. Brockway, S. D. Reynolds, and B. R. Stripp. Beta-catenin is not necessary for maintenance or repair of the bronchiolar epithelium. Am. J. Respir. Cell Mol. Biol. (Epub 2009).